毕业于大连理工大学，生物化工专业，工学博士学位，现为南京理工大学环境与生物工程学院副教授，2010-2011年英国利兹大学LIGHT实验室访问学者。
主要研究领域包括化合物的细胞毒理学研究、微生物对有毒化合物的降解、微生物育种和医学生物材料等，参加过“空间生物舱总体关键技术”（863）、“蜡状芽胞杆菌ATCC14579中赖氨酰tRNA合成酶Ⅰ（lysRSⅠ）和tRNA-other的生理功能研究”、等国家自然科学基金项目以及“复合微生物降解原油的研究”等多项横向课题的研究。
[1] Jizhong Wang, Jing Feng, Weile Li, Chengli Yang, Xing Chen, Bingxin Bao, Junfang Yang, Peng Wang, Dali Li, Ruofu Shi. Characterization of a novel (R)-mandelate dehydrogenase from Pseudomonas putida NUST506, Journal of Molecular Catalysis B: Enzymatic, 2015(120): 23-27
[2] Ruofu Shi, Peng Wang, Huaizhi Luo,Bingxin Bao,Dali Li1.Chinese teacher’s bilingual teaching strategies ,International Conference on Education and Social Development 2015.12. Nanjing.
[3] Jing Qi, Ruo-fu Shi, Jian-ming Yu, Yi Li, Sheng-tao Yuan, Ji-zhu Yang, Jiang-miao Hu, Ai-qun Jia.Chemical Constituents from Leaves of Camellia nitidissima and Their Potential Cytotoxicity on SGC7901 Cells, Chinese Herbal Medicines, 2016,8(1): 80-84
[4] Ruofu Shi, Zhaocai Zhong, Yunyun Gong ,Shaokang Wang, Guiju Sun.The genetic toxicity of fumonisin B1 on liver cell. International Conference on Energy and Environment Engineering. April.2015.Nanjing.China.
[5].Shao Kang Wang, Ting Ting Wang, Gui Ling Huang, Ruo Fu Shi, Li Gang Yang, Gui Ju Sun. Stimulation of the proliferation of human normal esophageal epithelial cells by fumonisin B1 and its mechanism. Experimental and Therapeutic Medicine, 7(1):55-60,2014.
[6].YuelinWu, Lingjian Zhu, Chunquan Sheng, Jianzhong Yao, Zhenyuan Miao, Wannian Zhang, Yunyang Wei, Ruofu Shi*. Synthesis and biological activities of fluorinated 10-hydroxycamptothecin and SN38. Journal of Fluorine Chemistry, 157, 48-51，2014.
[7]. Peng Wanga, Dali Li a, Junfang Yang, Lei Jiang, Jing Feng, Chengli Yang, Ruofu Shi. Immobilization of (S)-mandelate dehydrogenase and its catalytic performance on stereoselective transformation of mandelic acid. Journal of the Taiwan Institute of Chemical Engineers, 2014 (45):744–748.
[8]. 王少康，王婷婷，黄桂玲，石若夫，杨立刚，董淑楠，孙桂菊.伏马菌素B1对人正常食管上皮细胞细胞周期相关基因mRNA表达的影响.食品科学，2013（23）：313-316.
[9]Shi Ruofu, Sun Guiju, Wang Shaokang, Huang zongjun, Chen Guowei. Analysis for Possible Source(s) of Bacteria Contamination of Ready-to-Eat Meat Products in Processing and Selling in Big Supermarket of Nanjing, China. Conference on Environmental Pollution and Public Health, August, 2012, Shanghai, China.
[10].Shi Ruofu, Wang Shaokang, Chen Guowei, Sun Guiju. Controlling the Microbial Contamination of RTE Foods during Processing and Selling Using the Principle of HACCP in Big Supermarket of Nanjing, China. Conference on Environmental Pollution and Public Health, August, 2012, Shanghai, China.
参与国家级规划教材《植物基因工程》（第二版）（科学出版社978-7-03-0410990-0）的编写。
讲授硕士研究生课程：1.微生物应用技术；2.高级微生物实验技术